N.B. IT IS EXPECTED THAT RESEARCHERS ACKNOWLEDGE THE OLSON LAB WEBSITE AND/OR RELEVANT PUBLICATIONS WHEN USING THE DATA OR INFORMATION PROVIDED BELOW
Hymenolepis microstoma genome data
Data from the Hymenolepis microstoma genome sequencing effort at the Wellcome Trust Sanger Institute, based on specimens from our laboratory model, are made publicly available as the data are generated.
WEBSITE
Data from the Hymenolepis microstoma genome sequencing effort at the Wellcome Trust Sanger Institute, based on specimens from our laboratory model, are made publicly available as the data are generated.
WEBSITE
Sequence Alignments
NEXUS-formatted files with sites excluded from published analyses defined as character exclusion sets ('EXSET') in an 'Assumptions' block.
Riddiford N and PD Olson. 2011. Wnt gene loss in flatworms. Development Genes and Evolution 221:187-197 PDF
ALIGNMENT (ALL SITES)
ALIGNMENT (ANALYSED POSITIONS)
Olson PD, JN Caira, K Jensen, H Palm, RM Overstreet and I Beveridge. 2010. Evolution of the trypanorhynch tapeworms: parasite phylogeny supports independent lineages of sharks and rays. International Journal for Parasitology 40:223-242
ALIGNMENT | Supplementary Data S1 | PDF
Olson PD. 2008. Invited Review: Hox genes and the parasitic flatworms: new opportunities, challenges and lessons from the free-living. Parasitology International 57:8-17
ALIGNMENT* | PDF
(*Note: regions outside of the homeodomain used for analysis are not in alignment)
Olson PD, LG Poddubnaya, DTJ Littlewood and T Scholz. 2008. On the position of Archigetes and its bearing on the early evolution of the tapeworms. Journal of Parasitology 94:898-904
ALIGNMENT | PDF
Olson PD, TH Cribb, VV Tkach, RA Bray and DTJ Littlewood. 2003. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33:733-755
ALIGNMENT | PDF
NEXUS-formatted files with sites excluded from published analyses defined as character exclusion sets ('EXSET') in an 'Assumptions' block.
Riddiford N and PD Olson. 2011. Wnt gene loss in flatworms. Development Genes and Evolution 221:187-197 PDF
ALIGNMENT (ALL SITES)
ALIGNMENT (ANALYSED POSITIONS)
Olson PD, JN Caira, K Jensen, H Palm, RM Overstreet and I Beveridge. 2010. Evolution of the trypanorhynch tapeworms: parasite phylogeny supports independent lineages of sharks and rays. International Journal for Parasitology 40:223-242
ALIGNMENT | Supplementary Data S1 | PDF
Olson PD. 2008. Invited Review: Hox genes and the parasitic flatworms: new opportunities, challenges and lessons from the free-living. Parasitology International 57:8-17
ALIGNMENT* | PDF
(*Note: regions outside of the homeodomain used for analysis are not in alignment)
Olson PD, LG Poddubnaya, DTJ Littlewood and T Scholz. 2008. On the position of Archigetes and its bearing on the early evolution of the tapeworms. Journal of Parasitology 94:898-904
ALIGNMENT | PDF
Olson PD, TH Cribb, VV Tkach, RA Bray and DTJ Littlewood. 2003. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33:733-755
ALIGNMENT | PDF
Primers
Olson Lab Primers XLS
Includes PCR/RT-PCR/QPCR primer definitions for universal nuclear ribosomal, mitochondrial, Hox and other homeodomain genes in (mostly) parasitic flatworms, especially Hymenolepis microstoma.
Olson Lab Primers XLS
Includes PCR/RT-PCR/QPCR primer definitions for universal nuclear ribosomal, mitochondrial, Hox and other homeodomain genes in (mostly) parasitic flatworms, especially Hymenolepis microstoma.
Protocols
Working in the Olson Lab PDF
Working with RNA PDF
RNA Extraction Using RNeasy Spin Columns PDF
cDNA Synthesis Using SuperScript III PDF
PCR Amplification PDF
PCR Datasheet
Gel Electrophoresis PDF
PCR Purification Using QIAquick Spin Columns PDF
DNA/RNA Quantification Using Nanodrop PDF
Cloning Using Agilent Strataclone PDF
Synthesis of Digoxigenin (DIG)-labelled Riboprobes PDF
Preparation of 4% PFA Fixative PDF
Whole-Mount In Situ Hybridisation (Pat's guide with recipes) PDF
Whole-Mount In Situ Hybridisation (Nick's guide) PDF
Working in the Olson Lab PDF
Working with RNA PDF
RNA Extraction Using RNeasy Spin Columns PDF
cDNA Synthesis Using SuperScript III PDF
PCR Amplification PDF
PCR Datasheet
Gel Electrophoresis PDF
PCR Purification Using QIAquick Spin Columns PDF
DNA/RNA Quantification Using Nanodrop PDF
Cloning Using Agilent Strataclone PDF
Synthesis of Digoxigenin (DIG)-labelled Riboprobes PDF
Preparation of 4% PFA Fixative PDF
Whole-Mount In Situ Hybridisation (Pat's guide with recipes) PDF
Whole-Mount In Situ Hybridisation (Nick's guide) PDF
Information
Hymenolepis microstoma Handbook PDF
General information on their biology and in vivo development, including a guide to animal husbandry.
Hymenolepis microstoma Handbook PDF
General information on their biology and in vivo development, including a guide to animal husbandry.
RECENT PUBLICATIONS
Olson et al. 2020. Complete representation of a tapeworm genome reveals chromosomes capped by centromeres, necessitating a dual role in segregation and protection. BMC Biology PUBLICATIONS
James and Olson. 2020. the tapeworm interactive: inferring confidence scored protein-protein interactions from the proteome of Hymenolepis microstoma PUBLICATIONS
International Helminth Genomes Consortium. 2019. Comparative genomics of the major parasitic worms. Nature Genetics PUBLICATIONS
NEWS
Available Now! Next Generation Systematics 2016. PD Olson, J Hughes and JA Cotton (ed.). Cambridge University Press
NEWS
Olson et al. 2020. Complete representation of a tapeworm genome reveals chromosomes capped by centromeres, necessitating a dual role in segregation and protection. BMC Biology PUBLICATIONS
James and Olson. 2020. the tapeworm interactive: inferring confidence scored protein-protein interactions from the proteome of Hymenolepis microstoma PUBLICATIONS
International Helminth Genomes Consortium. 2019. Comparative genomics of the major parasitic worms. Nature Genetics PUBLICATIONS
NEWS
Available Now! Next Generation Systematics 2016. PD Olson, J Hughes and JA Cotton (ed.). Cambridge University Press
NEWS