Olson Lab

Department of Life Sciences, The Natural History Museum, London SW7 5BD United Kingdom

N.B. IT IS EXPECTED THAT RESEARCHERS ACKNOWLEDGE THE OLSON LAB WEBSITE AND/OR RELEVANT PUBLICATIONS WHEN USING THE DATA OR INFORMATION PROVIDED BELOW
Hymenolepis microstoma genome data

Data from the
Hymenolepis microstoma genome sequencing effort at the Wellcome Trust Sanger Institute, based on specimens from our laboratory model, are made publicly available as the data are generated.

WEBSITE
Sequence Alignments

NEXUS-formatted files with sites excluded from published analyses defined as character exclusion sets ('EXSET') in an 'Assumptions' block.

Riddiford N and PD Olson. 2011. Wnt gene loss in flatworms. Development Genes and Evolution 221:187-197 PDF
ALIGNMENT (ALL SITES)
ALIGNMENT (ANALYSED POSITIONS)

Olson PD, JN Caira, K Jensen, H Palm, RM Overstreet and I Beveridge. 2010. Evolution of the trypanorhynch tapeworms: parasite phylogeny supports independent lineages of sharks and rays. International Journal for Parasitology 40:223-242
ALIGNMENT |
Supplementary Data S1 | PDF

Olson PD. 2008. Invited Review: Hox genes and the parasitic flatworms: new opportunities, challenges and lessons from the free-living. Parasitology International 57:8-17
ALIGNMENT* | PDF
(*Note: regions outside of the homeodomain used for analysis are not in alignment)

Olson PD, LG Poddubnaya, DTJ Littlewood and T Scholz. 2008. On the position of Archigetes and its bearing on the early evolution of the tapeworms. Journal of Parasitology 94:898-904
ALIGNMENT | PDF

Olson PD, TH Cribb, VV Tkach, RA Bray and DTJ Littlewood. 2003. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33:733-755
ALIGNMENT | PDF
Primers

Olson Lab Primers XLS

Includes PCR/RT-PCR/QPCR primer definitions for universal nuclear ribosomal, mitochondrial, Hox and other homeodomain genes in (mostly) parasitic flatworms, especially Hymenolepis microstoma.

Protocols

Working in the Olson Lab PDF

Working with RNA
PDF

RNA Extraction Using RNeasy Spin Columns
PDF

cDNA Synthesis Using SuperScript III
PDF

PCR Amplification PDF

PCR
Datasheet

Gel Electrophoresis PDF

PCR Purification Using QIAquick Spin Columns PDF

DNA/RNA Quantification Using Nanodrop PDF

Cloning Using Agilent Strataclone PDF

Synthesis of Digoxigenin (DIG)-labelled Riboprobes PDF


Preparation of 4% PFA Fixative PDF

Whole-Mount In Situ Hybridisation (Pat's guide with recipes)
PDF

Whole-Mount In Situ Hybridisation (Nick's guide)
PDF
Information

Hymenolepis microstoma Handbook PDF

General information on their biology and in vivo development, including a guide to animal husbandry.
RECENT PUBLICATIONS

Olson PD, J Hughes and JA Cotton. 2016.
Next Generation Systematics. Cambridge University Press 356 p. PUBLICATIONS

Koziol et al. 2016.
Comparative analysis of Wnt expression identifies a highly conserved developmental transition in flatworms. BMC Biology PUBLICATIONS

Muehlenbachs et al. 2015.
Malignant transformation of Hymenolepis nana in a human host. New England Journal of Medicine PUBLICATIONS

NEWS

4th European Meeting on Planarian Biology, Sant Feliu de Guixols, Catalonia, Spain, 25-27 September

ISOMORPHOLOGY: An introduction to the art and science of Gemma Anderson

READ Alessandro Minelli’s commentary “The tapeworm’s elusive antero-posterior polarity” regarding our work published in BMC Biology.

BBC, NPR television and radio interviews regarding New England Journal of Medicine publication: “How did man die of cancer from a tapeworm?

NGS_COVER_FRONT

Available Now! Next Generation Systematics 2016. PD Olson, J Hughes and JA Cotton (ed.). Cambridge University Press
NEWS

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Now Accepting Submissions: "Planarians to Parasitism: Evolution, Development and Stem Cells in Flatworms" Special EvoDevo + Parasites & Vectors Thematic Volume READ